Determination of hepatic 3-hydroxy-3-methylglutaryl CoA reductase activity in man

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Determination of hepatic 3-hydroxy-3-methylglutaryl CoA reductase activity in man.

Procedures were developed for the determination of the activity of the microsomal enzyme 3-hydroxy-3-methylglutaryl CoA reductase (HMG CoA reductase, EC 1.1.1.34) in human liver. The enzyme assay could be carried out with as little as 20 mg of fresh liver tissue, thus making the method applicable to specimens obtained by percutaneous liver biopsy. Experiments were carried out to determine optim...

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The maturation status of dendritic cells determines whether interacting T cells are activated or if they become tolerant. Previously we could induce T cell tolerance by applying a 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) reductase inhibitor (HMGCRI) atorvastatin, which also modulates MHC class II expression and has therapeutic potential in autoimmune disease. Here, we aimed at elucidating the i...

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Improved synthesis of 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA).

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The statins are inhibitors of 3-hydroxy-3-methylglutaryl CoA (HMG-CoA) reductase (HMG-CoAR), and are utilized to decrease levels of atherogenic lipoproteins in patients with, or who are at high risk of, cardiovascular disease. This study describes the inhibition of a recombinant, catalytic fragment of human HMG-CoAR by a new statin, rosuvastatin (CRESTOR(R)). Binding is reversible and involves ...

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ژورنال

عنوان ژورنال: Journal of Lipid Research

سال: 1974

ISSN: 0022-2275

DOI: 10.1016/s0022-2275(20)36838-3